cryo 25°
The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

High resolution electron micrograph of vitreous section of keratin intermediate filaments in the midportion of stratum corneum of human epidermis.
The fine structure of the keratin filaments is well resolved and their molecular organisation is seen in favourable cases (inset).
Ashraf Al-Amoudi, Laboratoire d'Analyse Ultrastructurale, Lausanne.
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