cryo immuno
The first cryo knife with a diamond
platform, guarantees the best possible
sectioning for sucrose infiltrated samples
(Tokuyasu).
The diamond platform guarantees an
easy and gentle section pick-up.
The sections are collected directly from
the diamond surface using a loop and a
sucrose/methyl-cellulose droplet (W. Liou
et al., Histochemistry and Cell Biology,
Vol. 106, pp. 41 – 55, 1996. P. J. Peters
et al., Current Protocols in Cell Biology,
pp. 4.7.1 – 4.7.18, 2006).
The 35° angle leads to a considerable
reduction in mechanical stresses and
therefore to improved structure preservation in sucrose infiltrated samples (E. Bos
et al., Journal of Structural Biology 175,
pp. 62 – 72, 2011).
We recommend the cryo immuno knife
also for sectioning frozen hydrated
samples (CEMOVIS). The 35° angle is a
good compromise between durability
and cutting performance (A. Al-Amoudi
et al., Journal of Structural Biology 150,
pp. 109 – 121, 2005).
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Mouse optic nerve, immunolabeling of the major myelin protein proteolipid protein (PLP), 10 nm gold.
Wiebke Möbius, Dept. of Neurogenetics, EM Core Facility, MPI of Experimental Medicine, Göttingen.
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