 | cryo 25° | |
 | cryo immuno | |
| cryo 35°/45° | |
| cryotrim 20°/45° | |
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cryo immuno The first cryo knife with a diamond platform, guarantees the best possible sectioning for cryo-immuno-chemistry. The diamond platform guarantees easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet (Refs. Liou, Peters). The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples. |
  Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti-CD63 and protein-A gold 10nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam. |
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